map kinase antibody, activated  (NSJ Bioreagents)

Journal: Biomolecules & Therapeutics

Article Title: Cynaropicrin Induces Reactive Oxygen Species-Dependent Paraptosis-Like Cell Death in Human Liver Cancer Cells

doi: 10.4062/biomolther.2025.011

Figure Lengend Snippet: Alleviation of cynaropicrin-induced cytotoxicity through a p38 MAPK inhibitor in Hep3B cells. (A) After treating cells with cynaropicrin for the indicated duration, changes in the expression of the three types of MAPKs were examined via Western blot analysis. (B-D) Cells were pretreated with the p38 MAPK inhibitor SB203580 for 1 h and then treated with cynaropicrin for 24 h. Thereafter, changes in morphology (B), cell viability (C), and expression of the indicated proteins (D) were examined. (C) Numerical data are presented as mean ± SD (n=3). *** p <0.001 compared to untreated group; ### p <0.001 compared to cynaropicrin-treated group.

Article Snippet: Antibodies against poly(ADP-ribose) polymerase (PARP), apoptosis-linked gene 2-interacting protein X (Alix), p38 mitogen activated protein kinase (MAPK), extracellular signal-regulated kinase ( de Ridder et al ., 2023 ), c-Jun N-terminal kinases (JNK), phosphor (p)-JNK, and β-actin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).

Techniques: Expressing, Western Blot

Journal: Biomolecules & Therapeutics

Article Title: Cynaropicrin Induces Reactive Oxygen Species-Dependent Paraptosis-Like Cell Death in Human Liver Cancer Cells

doi: 10.4062/biomolther.2025.011

Figure Lengend Snippet: Effects of the p38 MAPK inhibitor on cynaropicrin-induced ER stress and mitochondrial impairment in Hep3B cells. The effects of SB203580 on the cynaropicrin-induced increase in intracellular Ca 2+ concentration (A, B), ER stress (C, D), and mitochondrial dysfunction (E, F) were evaluated via flow cytometry. (B, D, F) Numerical data are presented as mean ± SD (n=3). *** p <0.001 compared to untreated group; # p <0.05 and ### p <0.001 compared to cynaropicrin-treated group.

Article Snippet: Antibodies against poly(ADP-ribose) polymerase (PARP), apoptosis-linked gene 2-interacting protein X (Alix), p38 mitogen activated protein kinase (MAPK), extracellular signal-regulated kinase ( de Ridder et al ., 2023 ), c-Jun N-terminal kinases (JNK), phosphor (p)-JNK, and β-actin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).

Techniques: Concentration Assay, Flow Cytometry

Journal: Biomolecules & Therapeutics

Article Title: Cynaropicrin Induces Reactive Oxygen Species-Dependent Paraptosis-Like Cell Death in Human Liver Cancer Cells

doi: 10.4062/biomolther.2025.011

Figure Lengend Snippet: A schematic diagram illustrating the mechanisms by which cynaropicrin induces cell death in Hep3B cells, including the promotion of mitochondrial ROS production, activation of the p38 MAPK pathway, inhibition of Alix protein, and enhancement of ER stress.

Article Snippet: Antibodies against poly(ADP-ribose) polymerase (PARP), apoptosis-linked gene 2-interacting protein X (Alix), p38 mitogen activated protein kinase (MAPK), extracellular signal-regulated kinase ( de Ridder et al ., 2023 ), c-Jun N-terminal kinases (JNK), phosphor (p)-JNK, and β-actin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).

Techniques: Activation Assay, Inhibition